Human CRISPR Metabolic Gene Knockout Library

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Human CRISPR Metabolic Gene Knockout Library

Human CRISPR Metabolic Gene Knockout Library

Catalog# LIBR-H005-P002 LIBR-H005-P100 LIBR-H005-P200 LIBR-H005-P500

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Instruction

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The CRISPR knockout library targets 2,981 human metabolic genes with a total of 30,290 knockout plasmid vectors, of which 10 different gRNA vectors are designed for each gene, in addition to 500 control vectors targeting intergenic sequences. The library uses YKO-LV004 as the backbone, which is a two-plasmid system that expresses only gRNA, while the Cas9 gene is on a separate vector that needs to be used in conjunction.
Product Name
Human CRISPR Metabolic Gene Knockout Library
Species
Human
Library Type
Knockout Library
Plasmid System
Dual-plasmid System
Virus Packaging System
3rd Lentivirus Packaging System
Targeted Genes
2981
gRNA Number
30290
Non-targeting gRNA Number
500
Selection Marker
Puro
Backbone Map
YKO-LV004 vector formula
Click to view the full image
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
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Cas9 Stable Cell Line
The Cas9 cell lines in our cell bank can stably express Cas9 protein. So gene knockout can be achieved by transfecting gRNA. Simultaneously transfecting gRNA and donor DNA can achieve gene knock-in/point mutation, effectively improving experimental efficiency.
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